Cytokine Assay

KOMABIOTECH is applying Luminex analysis system for cytokine service.
In the Luminex system different analytes (in case of cytokine simultaneous analysis of up to 40 kinds are possible) in a sample of one drop (10 ~ 50ul) can be analyzed simultaneously in a fast and simple way. And to obtain the standard curve for each substance quantitative analysis compares each cytokine for this analysis because it can get reliable results.
■ Advantages:
• Get reliable result rapidly (~ 2 days)
With small amount of sample (10 ~ 50 ul/sample), various analyzes are possible.
A single sample processing allows simultaneous analysis of various proteins
Can be measured in various specimens such as Serum / Plasma, cell culture supernate.

■ Measuring principle
• Bioassay technology: Like the specific binding of the antigen- antibody used in the ELISA analysis or the complementary  coupling reaction of the DNA used in DNA chip analysis is used for specific binding of the cytokines to a particular substance to be analyzed in the sample (serum,etc.)
Bead using technology: A bead size of 5.6um is used. 100 beads are distinguished by different fluorescent dyes and the luminex device recognizes each different fluorescent dye as a particular number. When measuring each bead through a luminex device (when reading), the luminex device will be able to distinguish the measurements by the bead's unique number. When you attach a specific antibody to a certain number of bead and mix the various beads the sample do the reading, the instrument can measure the response level of each number of bead , so you can measure 100 reactions simultaneously.
Fluidic system: This measurement is a fast data processing that can read about 1,000 beads per second can be read to be able to analyze the reactions of several substances in a reaction solution at the same time.

■ Deliverables:
• Luminex Measure values[MFI] raw data .[xls]
• The standard curve for each measured cytokine [pdf]
• Quantitative analysis results for each sample (duplicate or group average, standard deviation)
• The actual well map history of experimented samples.
• The protocol of each analysis
• Remaining reagents and samples (upon request)