관리용-Forensic Identification

 

Product Name EzWay Direct F PCR Kit
Cat. No. K0569000
Price Please inquire
Size 100T
Description
A major limitation of Polymerase Chain Reaction (PCR)-based research and diagnostic and forensic tests is the inhibition of DNA polymerases by many substances like as anticoagulants (e.g., heparin, EDTA, sodium citrate) and heme group, proteins, polysaccharides, cell debris, and charged inorganic compounds, etc. Therefore, DNA is usually extracted from specimen according to phenol-chloroform method or by an alternative ‘silica-membrane filter’ method. However, this is time consuming and labor intensive and there is also a risk of introducing contamination during the preparation.

EzWay Direct F PCR allows direct PCR from buccal or blood FTA disc, blood or hair lysate without any DNA purification steps just by immersing the FTA punch or pipetting lysate into PCR mixture. EzWay Direct F Buffer contains the proper amounts of buffer, MgCl2, dNTPs and the components that facilitate direct PCR amplification, but hot start enzyme is supplied with separate tube for the extended activity
Specification
• Direct PCR from buccal or blood FTA disc, blood or hair lysate without any DNA purification steps just by immersing the FTA punch or pipetting lysate into PCR mixture
Components
• EzWay Direct F Buffer Mix 100T (25uL PCR)
• Hot Taq-fx (5units/uL)
• 25x Inhibitor cocktail
Platform
• Applied Biosystems 310 Genetic Analyzer filled with POP-4 & 36cm capillaries
• ABIPRISM® 3100/3100-Avant Genetic Analyzer filled with POP-4 & 36cm capillaries
• Applied Biosystems 3130/3130xl Genetic Analyzer filled with POP-4 & 36cm capillaries
• GeneAmp® PCR System 9600/9700/Veriti
Applications
Human identification with a forensic sample
Citation