DNA Purification

 

Product Name EzWay Genomic DNA Kit, Blood
Cat. No. K11741
Price Please inquire
Size 200T
Description
EzWay™ Genomic DNA Kit (Blood) is an excellent tool offering a speedy and economic method to purify total DNA (e.g. genomic, mitochondrial and viral DNA) from whole blood (fresh or frozen), plasma, serum, buffy coat, body fluids, lymphocytes and cultured cells. This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica-based membranes, washes DNA with ethanol-contained Wash Buffer and then elutes purified DNA by low salt Elution Buffer or ddH2O. Compare with other harmful and time-consuming procedures, such as phenol/chloroform extraction and ethanol precipitation, After using this kit, the high quality total DNA can be used directly for the downstream applications.
Specification
• Binding Capacity: 45ug
• Column Capacity: 800ul
• Recovery Rate: 85-95%
Components
• Binding Buffer
• Wash Buffer A
• Wash Buffer B
• Elution Buffer
• Proteinase K
• DNA-prep Column
• 2ml Collection Tube
• Elution Tube
Principle
Genomic DNA released from whole blood treated by lysis buffer is bound to a silica gel membrane in the presence of high concentration of chaotropic salt. The purified genomic DNA on the membrane is eluted in the elution buffer.
Applications
• PCR amplifications
• Membrane hybridizations
• Restriction enzyme digestion
Citation