DNA Purification

 

Product Name EzWay Total RNA Isolation Kit
Cat. No. K11751
Price Please inquire
Size 200T
Description
EzWay™ Total RNA Isolation Kit is designed for extraction of total RNA from animal tissue and cultured cells. Some specially modified protocols are developed for other samples, such as bacteria and yeast. This method first lyses cells by using a chaotropic salt, then binds RNA to silica-based membranes, washes RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free ddH2O. It takes 30 min for an entire procedure, and the purified RNA is ready for RT-PCR, northern blotting, primer extension and cDNA library construction.
Specification
• Binding Capacity: 45ug
• Column Capacity: 800ul
• Recovery Rate: 85-95%
• Yield: 12ul
• A260/A280 : 2
Components
• Lysis Buffer
• Wash Buffer A
• Wash Buffer B
• DEPC-treated water
• RNA-prep Column
• 2 ml Collection Tube
• Elution Tube
Principle
RNA released from tissue by chaotropic salt-containing lysis buffer is bound to silica-based membranes. The purified RNA on the membrane is eluted with RNase-free ddH2O.
Applications
• RT-PCR
• Northern blotting
• Primer extension
• cDNA library construction
Citation
The prophylactic effect of probiotic Bacillus polyfermenticus KU3 against cancer cells
NK Lee, SH Son, EB Jeon, GH Jung, JY Lee, HD Paik
Journal of Functional Foods, 2015

Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters
NK Lee, SY Kim, KJ Han, SJ Eom, HD Paik
LWT-Food Science and Technology, 2014