DNA Purification

 

Product Name EzWay PCR Clean-Up Kit
Cat. No. K11761
Price Please inquire
Size 200T
Description
EzWay™ PCR Clean-Up Kit is designed for rapid purification of DNA and removal of contaminants. It provides a fast and simple method using advanced silica-based membrane technology for rapid and efficient recovery of DNA fragments free of contaminants such as oligonucleotides, nucleotides, and polymerase in yields exceeding up to 95%. The silica-based membrane is uniquely adapted to isolate DNA from aqueous solutions. The purified DNA is suitable for a variety of applications such as restriction enzyme digestion, labeling, ligation, transformation, and sequencing reactions.
Specification
• Sample Amount: up to 100 ul • PCR product or enzymatic reaction product
• Recovery: 85% - 95%
• Operation time: within 15 min
• Elution volume: 40 ul
Components
• DNA-prep Column
• 2 ml Collection Tube
• Elution Tube
• Binding Buffer
• Wash Buffer
• Elution Buffer
Principle
After addition of DNA binding buffer to PCR sample, PCR products are bound to a silica gel membrane in the presence of high concentration of chaotropic salt. The purified PCR product on the membrane is eluted in elution buffer.
Applications
• Sequencing
• Ligation
• Digestion
• Labeling
• Transformation etc.
Citation
Prevalence of Mycobacterium avium subsp. paratuberculosis and Escherichia coli in blood samples from patients with inflammatory bowel disease
N Nazareth, F Magro, E Machado, TG Ribeiro
Medical Microbiology and Immunology 2015