DNA Purification

 

Product Name EzWay Gel Extraction Kit
Cat. No. K11771
Price Please inquire
Size 200T
Description
EzWay™ Gel Extraction Kit is designed for rapid purification of DNA from standard agarose gels. It provides a fast and simple method using advanced silica-based membrane technology for rapid and efficient purification of 100bp-10kb DNA fragments. DNA adsorbs to the silica-membrane in the presence of high salt while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with Elution Buffer or water. The purified DNA is suitable for a variety of applications such as fluorescent DNA sequencing, transformation, restriction mapping, cloning, and labeling.
Specification
• Sample Amount:up to 200 mg of agarose gel
• Recovery: 70% - 85%
• Operation time: within 20 min
• Elution volume: 40 ul
Components
• DNA-prep Column
• Collection Tube
• Elution Tube
• Extraction Buffer
• Wash Buffer
• Elution Buffer
Principle
After addition of Extraction Buffer to agarose gel slice sample and heating at 50-55°C to melt gel, DNA is bound to a silica gel membrane in the presence of high concentration of chaotropic salt. The purified DNA product on the membrane is eluted in elution buffer.
Applications
• Fluorescent DNA sequencing
• Ligation
• Transformation
• PCR
• Micro-injection
• DNA microarry-based analysis
• Restriction mapping
• Labeling etc.
Citation
Molecular and Physiological Mechanisms of Heavy Metal Tolerance in Atriplex halimus
R El-Bakatoushi, AM Alframawy, A Tammam
International Journal of Phytoremediation 2015

Further Molecular Characterization and Effect on Host Photosynthetic Pigments and Carbohydrate Pools of an Egyptian Isolate of TBSV
FA Fattouh, AS Ali, RM Fathy
J Plant Pathol Microb, 2015