PCR Reagent

 

Product Name Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)
Cat. No. K0561005
Price Please inquire
Size 500U
Description
Taq DNA Polymerase is the enzyme from the cloned Thermus aquaticus expressed in E.coli. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium and also possesses a 5'→3' exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Specification
• Ultrapure, thermostable recombinant enzyme
• Highly thermostable: 10min at 97°C, 60min at 94°C
• 5'→3' exonuclease activity
• Extra A addition activity
• Amplification range:
- Lambda DNA: up to 6Kb (Max. 12Kb)
- Human genomic DNA: up to 2Kb (Max. 4Kb)
• Composition of Taq polymerase and Taq PCR buffer without MgCl2
• No Nuclease, RNase, Protease contamination
Components
• Taq DNA Polymerase II, 500U
• 10X Taq PCR Buffer II (MgCl2 free)
• MgCl2 Stock solution
• dNTP Mix (2.5mM each)
• 10X Loading Buffer
• Nuclease Free Water
• 4X Magic Buffer
Citation