PCR Reagent

 

Product Name Taq DNA Polymerase Kit I
Cat. No. K0561002
Price Please inquire
Size 500U
Description
Taq DNA Polymerase is the enzyme from the cloned Thermus aquaticus expressed in E.coli. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium and also possesses a 5'→3' exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Specification
• Ultrapure, thermostable recombinant enzyme
• Highly thermostable: 10min at 97°C, 60min at 94°C
• 5'→3' exonuclease activity
• Extra A addition activity
• Amplification range:
- Lambda DNA: up to 6Kb (Max. 12Kb)
- Human genomic DNA: up to 2Kb (Max. 4Kb)
• Composition of Taq polymerase and Taq PCR buffer containing MgCl2
• No Nuclease, RNase, Protease contamination
Components

• Taq DNA Polymerase I, 500U
• 10X Taq PCR Buffer I (with MgCl2)
• dNTP Mix (2.5mM each)
• 10X Loading Buffer
• Nuclease Free Water
• 4X Magic Buffer (for high GC content)
Citation
The gene–diet interaction, LPL Pvu II and Hind III and carbohydrate, on the criteria of metabolic syndrome: KMSRI-Seoul Study
Y Kim, M Lee, Y Lim, Y Jang, HK Park, Y Lee
Nutrition, 2013

Supplementation of Korean fermented soy paste doenjang reduces visceral fat in overweight subjects with mutant uncoupling protein-1 allele
M Lee, S Chae, Y Cha,
Nutrition Research, 2012

The association of lipoprotein lipase PvuII polymorphism and niacin intake in the prevalence of metabolic syndrome: a KMSRI-Seoul study
E Shin, NY Park, Y Jang, H Oh, J Jeong, Y Lim
Genes & Nutrition, 2011

Characterization of the Chaetoceros salsugineum nuclear inclusion virus coat protein gene
YJ Park, SE Jung, Y Tomaru, WB Choi, YH Kim, H Mizumoto, K Nagasaki and TJ Choi
Virus Research, Volume 142, Issues 1-2, June (2009) Pages 127-133

Novel Molecular Mechanism for Actinomycin D Activity as an Oncogenic Promoter G-Quadruplex Binder
Hyun-Jin Kang and Hyun-Ju Park
Biochemistry, 2009, 48 (31), pp 7392-7398

Stromal Cell-Derived Factor-1 Promotes Myeloma Cell Growth in Both Autocrine and Paracrine Manners
Seong-Woo Kim, Ha-Yon Kim, Hyo-Jin Lee, M.D., Hwan-Jung Yun, M.D., Samyong Kim, M.D. and Deog-Yeon Jo, M.D.
Korean J Hematol. 2008 Sep;43(3):127-137

Possible association between serotonin transporter promoter region polymorphism and impulsivity in Koreans.
JH Lee, HT Kim, DS Hyun
Psychiatry Research, Volume 118, Issue 1, Pages 19-24, 2003