관리용-Detection Kit

 

Product Name EzWay Commassie Protein Assay Kit
Cat. No. K15210
Price Please inquire
Size 1kit
Description
EzWay™ Coomassie Protein Assay Kit provides a quick coomassie-binding, colorimetric method for total protein quantitation. When coomassie dye protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Performing the assay in either test tube or microplate format is simple: Combine a small amount of protein sample with the essay reagent, mix well, incubate briefly and measure the absorbance at 595 nm. Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, which are assayed alongside the unknown sample. When protein combined with Coomassie, they will arrive at balance within 2-5 min, the complete reaction is very quick, and the combo could maintain stability for 1 hour at room temperature whose senstivity is as 4 times as Lowry method. This kit can measure microgram of protein content and the test range of protein concentrations is 0-1500μg.
Specification
• Quick coomassie-binding, colorimetric method
• 4 times higher sensitivity as Lowry method
• Can measure microgram of protein content
• Test range of protein concentrations : 0-1500μg
Components
• Coomassie Assay Reagent 100ml
• BSA Standard (10mg/vial) 5 vials
Citation
Determination of Protein Concentration Using Bradford Microplate Protein Quantification Assay
RV Nouroozi, MV Noroozi, M Ahmadizadeh
International Electronic Journal of Medicine. 2015 Autumn; 4(1)