Detection Kit

 

Product Name EzWay BCA Protein Assay Kit
Cat. No. K15220
Price Please inquire
Size 1kit
Description
EzWay™ BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion.
Specification
• Sample Type : Serum, Plasma, Cell culture extracts, Tissue Extracts
• Technique : Solution-based detection of spectral absorbance at 562 nm; Smith Protein assay
• Detection Method : Colorimetric/Spectroscopic
• Assay Purpose : Measure protein concentration in solution
• Assay Range : 0.5 – 20 μg/ml
• Incubation conditions 60 min at 60°C
• Applications : Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays
Components
• BCA Reagent A 100ml
• BCA Reagent B 5ml
• Albumin Standard Ampules, 10mg x 5ea
Citation