A major limitation of Polymerase Chain Reaction (PCR)-based research and diagnostic and forensic tests is the inhibition of DNA polymerases by many substances like as anticoagulants (e.g., heparin, EDTA, sodium citrate) and heme group, proteins, polysaccharides, cell debris, and charged inorganic compounds, etc. Therefore, DNA is usually extracted from specimen according to phenol-chloroform method or by an alternative ‘silica-membrane filter’ method. However, this is time consuming and labor intensive and there is also a risk of introducing contamination during the preparation.
EzWay Direct F PCR allows direct PCR from buccal or blood FTA disc, blood or hair lysate without any DNA purification steps just by immersing the FTA punch or pipetting lysate into PCR mixture. EzWay Direct F Buffer contains the proper amounts of buffer, MgCl2, dNTPs and the components that facilitate direct PCR amplification, but hot start enzyme is supplied with separate tube for the extended activity