EzWay BCA Protein Assay Kit / K15220 - Detection Kit

Detection Kit

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Description
EzWay™ BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion.
Specification
• Sample Type : Serum, Plasma, Cell culture extracts, Tissue Extracts • Technique : Solution-based detection of spectral absorbance at 562 nm; Smith Protein assay • Detection Method : Colorimetric/Spectroscopic • Assay Purpose : Measure protein concentration in solution • Assay Range : 0.5 – 20 μg/ml • Incubation conditions 60 min at 60°C • Applications : Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays
Components
• BCA Reagent A 100ml • BCA Reagent B 5ml • Albumin Standard Ampules, 10mg x 5ea
Citation

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