DNA Purification
| Product Name | EzWay PCR Clean-Up Kit |
|---|---|
| Cat. No. | K11761 |
| Price | Please inquire |
| Size | 200T |
| Description |
|---|
EzWay™ PCR Clean-Up Kit is designed for rapid purification of DNA and removal of contaminants. It provides a fast and simple method using advanced silica-based membrane technology for rapid and efficient recovery of DNA fragments free of contaminants such as oligonucleotides, nucleotides, and polymerase in yields exceeding up to 95%. The silica-based membrane is uniquely adapted to isolate DNA from aqueous solutions. The purified DNA is suitable for a variety of applications such as restriction enzyme digestion, labeling, ligation, transformation, and sequencing reactions. |
| Specification |
• Sample Amount: up to 100 ul • PCR product or enzymatic reaction product |
| Components |
• DNA-prep Column |
| Principle |
After addition of DNA binding buffer to PCR sample, PCR products are bound to a silica gel membrane in the presence of high concentration of chaotropic salt. The purified PCR product on the membrane is eluted in elution buffer. |
| Applications |
• Sequencing |
| Citation |
| ■ Prevalence of Mycobacterium avium subsp. paratuberculosis and Escherichia coli in blood samples from patients with inflammatory bowel disease N Nazareth, F Magro, E Machado, TG Ribeiro Medical Microbiology and Immunology 2015 |